International Journal of Health and Life Sciences

Published by: Neoscriber Demo Publisher

Pichia pastoris as One of the Best Choice for Expression of Biopharmaceutical Proteins

Masoud Keikha ORCID 1 , *
Author Information
1 Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
2 Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Article information
  • International Journal of Health and Life Sciences: 5 (1); e87190
  • Published Online: January 13, 2019
  • Article Type: Letter
  • Received: December 8, 2018
  • Revised: January 6, 2019
  • Accepted: January 8, 2019
  • DOI: 10.5812/ijhls.87190

To Cite: Keikha M. Pichia pastoris as One of the Best Choice for Expression of Biopharmaceutical Proteins, Int J Health Life Sci. Online ahead of Print ; 5(1):e87190. doi: 10.5812/ijhls.87190.

Copyright © 2019, International Journal of Health and Life Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
  • 1. Vogl T, Hartner FS, Glieder A. New opportunities by synthetic biology for biopharmaceutical production in Pichia pastoris. Curr Opin Biotechnol. 2013;24(6):1094-101. doi: 10.1016/j.copbio.2013.02.024. [PubMed: 23522654]. [PubMed Central: PMC3841573].
  • 2. Potvin G, Ahmad A, Zhang Z. Bioprocess engineering aspects of heterologous protein production in Pichia pastoris: A review. Biochem Eng J. 2012;64:91-105. doi: 10.1016/j.bej.2010.07.017.
  • 3. Li P, Anumanthan A, Gao XG, Ilangovan K, Suzara VV, Duzgunes N, et al. Expression of recombinant proteins in Pichia pastoris. Appl Biochem Biotechnol. 2007;142(2):105-24. [PubMed: 18025573].
  • 4. Goodman M. Market watch: Sales of biologics to show robust growth through to 2013. Nat Rev Drug Discov. 2009;8(11):837. doi: 10.1038/nrd3040. [PubMed: 19876035].
  • 5. Cregg JM, Cereghino JL, Shi J, Higgins DR. Recombinant protein expression in Pichia pastoris. Mol Biotechnol. 2000;16(1):23-52. doi: 10.1385/MB:16:1:23. [PubMed: 11098467].
  • 6. Prielhofer R, Cartwright SP, Graf AB, Valli M, Bill RM, Mattanovich D, et al. Pichia pastoris regulates its gene-specific response to different carbon sources at the transcriptional, rather than the translational, level. BMC Genomics. 2015;16:167. doi: 10.1186/s12864-015-1393-8. [PubMed: 25887254]. [PubMed Central: PMC4408588].
  • 7. Darby RA, Cartwright SP, Dilworth MV, Bill RM. Which yeast species shall I choose? Saccharomyces cerevisiae versus Pichia pastoris (review). Methods Mol Biol. 2012;866:11-23. doi: 10.1007/978-1-61779-770-5_2. [PubMed: 22454110].
  • 8. Kamal S, Khan SU, Muhammad N, Shoaib M, Omar M, Pascal K, et al. Insights on heterologous expression of fungal cellulases in Pichia pastoris. Biochem Mol Biol. 2018;3(1):15. doi: 10.11648/j.bmb.20180301.13.

Featured Image:

Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .
Readers' Comments